Figure 1. The structure of New TAL.
We design seven new sticky ends which get the least score when judging the similarity.
Our parts can be connected in the right order by golden gate method.
New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.
New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE. The TALE can recognize 14bp DNA sequence:
Seven sticky ends:
AAAA AGGG GTAC GCTC TTTT TCGA CCCC
If you want to know how we design these ends, please go to see our project-Part3 TAL improve.
Usage and Biology
Because our TAL improvement is based on the 2012 Freiburg's project, you can just follow 2012 Freiburg's protocol to connect whole TALE.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 27
Illegal NotI site found at 489
- 21Illegal BamHI site found at 1
Illegal BamHI site found at 463
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC