Composite

Part:BBa_K1453408

Designed by: Yangyang Li, Binhan Hao, Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-17)

pykF-TAL1-His Tag

This part is used in our application test free in the cytoplasm. See more details please view iGEM14_SJTU_BioX_Shanghai.


Structure

3408.png

Figure 1 pykF-TAL1-His Tag

This part is based on the BBa_1453006 and BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1027
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1312
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1933
    Illegal BsaI.rc site found at 2545


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Categories
Parameters
None