This part is used in our application test on the membrane. See more details please view iGEM14_SJTU_BioX_Shanghai.
Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag
This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:18.104.22.168) or pykF.
Sequence and Features
- 10Illegal PstI site found at 2347
- 12Illegal PstI site found at 2347
- 21Illegal XhoI site found at 2062
- 23Illegal PstI site found at 2347
- 25Illegal PstI site found at 2347
Illegal AgeI site found at 862
- 1000Illegal BsaI site found at 483
Illegal BsaI.rc site found at 2968
Illegal BsaI.rc site found at 3580