Generator

Part:BBa_K145276

Designed by: Nathalie Busschaert   Group: iGEM08_KULeuven   (2008-09-07)


T7 polymerase generator under TetR repressible promoter

This device is part of a filter system. When there is no TetR the key will be generated. These keys will then open the lock and this will lead to the production of T7 DNA polymerase. When TetR is present, there will be no production of T7 DNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 413
    Illegal NheI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 60


[edit]
Categories
Parameters
None