Intermediate

Part:BBa_K145268

Designed by: Nathalie Busschaert   Group: iGEM08_KULeuven   (2008-09-07)


C2 P22 generator controlled by tetracyclin

Normally, tetR is produced and the production of C2 P22 is switched off. By adding tetracyclin (or anhydrotetracyclin) tetR is inactivated and C2 P22 is generated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1136


[edit]
Categories
Parameters
None