Project

Part:BBa_K1452002

Designed by: Keoni Gandall   Group: iGEM14_LA_Biohackers   (2014-11-02)


Pos/neg selection (Bacillus)

This is a positive/negative selection cassette for Bacillus. If flanked by Bacillus integration sites and integration sites for any chosen DNA, that DNA can be integrated into Bacillus and then the selection cassette can be integrated over by whatever DNA you chose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2585


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Categories
Parameters
None