Generator

Part:BBa_K1448005

Designed by: yuma kobayashi   Group: iGEM14_KIT-Kyoto   (2014-10-16)


ERG20 generator

This part contains lactose inducible expression cassette (BBa_K314103) and farnesyl-phosphate synthase from yeast [Erg20] (BBa_K849001). The transformants with this device can produce farnesyl-pyrophosphate (FPP) and geranyl-pyrophosphate (GPP) from isopentenyl-pyrophosphate (IPP) and dimethylallyl-pyrophosphate (DMAPP). However, because GPP is a by-product with synthesis of FPP, the volume of GPP is so smaller than those of FPP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None