dcmR gene under ATC inducible control
dcmR is the gene coding for the regulatory protein DcmR which regulates dcmA expression and binds DCM.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 1694
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1168
We cloned this part with an mCherry fusion at the C terminus of the dcmR gene in the pOXON-1 plamsid (pME6010 plasmid with kanamycin resistance).Expression was induced with various amounts of ATC and the following fluorescence data acquired. As shown in the figure below, mCherry fluorescence (arbitrary units) increases with amount of ATC used confirming that the dcmR gene was expressed under the control of the tet promoter and operator system.
For experimental details, see our experience page on this part.