Composite

Part:BBa_K1446003

Designed by: Philipp Lorenz   Group: iGEM14_Oxford   (2014-09-29)

dcmR gene under ATC inducible control

dcmR is the gene coding for the regulatory protein DcmR which regulates dcmA expression and binds DCM.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168


We cloned this part with an mCherry fusion at the C terminus of the dcmR gene in the pOXON-1 plamsid (pME6010 plasmid with kanamycin resistance).Expression was induced with various amounts of ATC and the following fluorescence data acquired. As shown in the figure below, mCherry fluorescence (arbitrary units) increases with amount of ATC used confirming that the dcmR gene was expressed under the control of the tet promoter and operator system.

Oxford Comparison of mean flouresence intensity from DcmR-mCherry expression induced at varying amounts of ATC.png

For experimental details, see our experience page on this part.

[edit]
Categories
Parameters
None