Part:BBa_K1444010
Composite promoter and weak B. subtilis RBS - C1-434
A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by the copies of the repressible promoter C1-434 BBa_R0052, and ending with the weak RBS from B. subtilis BBa_K780002.
This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.
IMPORTANT NOTE: Our experience with this entire series of repressible promoters suggests that they are unstable in the standard biobrick vector pSB1C3. As such, they have been submitted to the registry in a pUC57 vector BBa_K1444027.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |