Regulatory

Part:BBa_K1444007

Designed by: Dan Ziemianowicz   Group: iGEM14_Calgary   (2014-10-08)

Composite promoter and weak B. subtilis RBS - C2P22

A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by the repressible promoter C1P22 BBa_R0053,and ending with the weak RBS from B. subtilis BBa_K780002.

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C2P22 repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.

IMPORTANT NOTE: Our experience with this entire series of repressible promoters suggests that they are unstable in the standard biobrick vector pSB1C3. As such, they have been submitted to the registry in a pUC57 vector BBa_K1444027.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None