Regulatory

Part:BBa_K1444001

Designed by: Dan Ziemianowicz   Group: iGEM14_Calgary   (2014-10-08)

Composite promoter and weak B. subtilis RBS - pLacI x3

A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by three copies of the repressible promoter pLacI BBa_R0010, and ending with the weak RBS from B. subtilis BBa_K780002.

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

IMPORTANT NOTE: Our experience with this entire series of repressible promoters suggests that they are unstable in the standard biobrick vector pSB1C3. As such, they have been submitted to the registry in a pUC57 vector BBa_K1444027.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None