Primer

Part:BBa_K1443003

Designed by: Lassi Vapaakallio   Group: iGEM14_Aalto-Helsinki   (2014-10-09)

RFP Binding Forward Primer for Backbone Amplification

With this primer and its reverse counterpart BBa_K1443004 you can amplify backbones that have RFP as the insert using PCR. The forward primer binds to the end of the RFP coding sequence. These primers don't have long palindromic sites with each other.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None