Regulatory

Part:BBa_K1442025

Designed by: Yin Ho Vong   Group: iGEM14_Warwick   (2014-08-28)


Theophylline Aptazyme

The aptazyme is a RNA sequence that can be placed into a construct for design purposes (e.g. safety, regulatory), the aptazyme self-cleaves in the presence of theophylline to destroy the construct, preventing translation, transcription etc.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 137
    Illegal PstI site found at 151
    Illegal NotI site found at 7
    Illegal NotI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 137
    Illegal PstI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None