Primer

Part:BBa_K1441014

Designed by: Matthew Brewer   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase Ecor1 F primer

This primer is specific to the Bio-bricks suffix preceding the DNA Ligase insert(BBa_K525710). This primer was designed to remove the stop codon present in the standardized iGEM prefixes. This allows for proper insertion and expression of the insert into pGAPz vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None