Coding

Part:BBa_K1441013

Designed by: Matthew Brewer   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase from Escherichia coli with His-tag INSERT

This part, DNA ligase From E. Coli, was extracted from an IGEM regiserty PSB1C3 vector (BBa_k525710). This part contains on the insert with altered iGEM prefix and suffixes to allow for insertion into pGAP A vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


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Categories
Parameters
None