Plasmid

Part:BBa_K1441008

Designed by: Shivani Shah   Group: iGEM14_Georgia_State   (2014-10-06)


PGAPz alpha A + RFP

We started PGAPz alpha A with modified MCS and ligated it with RFP (iGEM registry parts) after digesting both with ECORI and PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 760
    Illegal suffix found in sequence at 1851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 760
    Illegal SpeI site found at 1852
    Illegal PstI site found at 1866
    Illegal NotI site found at 766
    Illegal NotI site found at 1859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 760
    Illegal BglII site found at 1
    Illegal BamHI site found at 2284
    Illegal XhoI site found at 736
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 760
    Illegal suffix found in sequence at 1852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 760
    Illegal XbaI site found at 775
    Illegal SpeI site found at 1852
    Illegal PstI site found at 1866
    Illegal NgoMIV site found at 3041
    Illegal NgoMIV site found at 3102
    Illegal AgeI site found at 1562
    Illegal AgeI site found at 1674
    Illegal AgeI site found at 2003
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2267
    Illegal BsaI.rc site found at 2466
    Illegal BsaI.rc site found at 3463
    Illegal SapI site found at 244


[edit]
Categories
Parameters
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