DNA

Part:BBa_K1441000

Designed by: Julia Dave,   Group: iGEM14_Georgia_State   (2014-09-22)


Mambalgin-1 (Analgesic)

Mambalgin (~200bp) exhibits analgesic properties that are similar in nature to morphine but has been shown not to carry the same dependency and withdrawal issues. The protein is naturally found in the African Black Mamba snake venom. Due to its reptilian origin the Mambalgin must be expressed in a Eukaryotic cell so that the necessary post-translational modifications can take place. The Mambalgin insert has been optimized for the pGAPzα vector system which is compatible with the yeast Pichia pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aMambalgin-1 exhibits analgesic properties