Coding

Part:BBa_K1440001

Designed by: Xuanye Cao   Group: iGEM14_Fudan   (2014-10-31)

Cre protein with ERT2 sequence


This biobrick is used to express Cre-ERTII protein. Cre when is binding the ERT2 sequence together, it will be not activated until tamoxifen inducing it.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 279
    Illegal XbaI site found at 2259
    Illegal PstI site found at 463
    Illegal PstI site found at 634
    Illegal PstI site found at 2443
    Illegal PstI site found at 2614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 463
    Illegal PstI site found at 634
    Illegal PstI site found at 2443
    Illegal PstI site found at 2614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 413
    Illegal BglII site found at 2393
    Illegal BamHI site found at 1286
    Illegal XhoI site found at 1960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 279
    Illegal XbaI site found at 2259
    Illegal PstI site found at 463
    Illegal PstI site found at 634
    Illegal PstI site found at 2443
    Illegal PstI site found at 2614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 279
    Illegal XbaI site found at 2259
    Illegal PstI site found at 463
    Illegal PstI site found at 634
    Illegal PstI site found at 2443
    Illegal PstI site found at 2614
    Illegal AgeI site found at 972
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 313
    Illegal BsaI site found at 2293


[edit]
Categories
Parameters
None