Part:BBa_K1439004

TAT-H4

Histone H4 can efficiently mediate gene transfer, it rich in positively charged alkaline amino acids and has the ability to combine with DNA. TAT-PTD contains an 11-amino acid peptide, and these amino acids YGRKKRRQRRR are highly basic, which is crucial for penetrating the plasma membrane. We use linker to link H4 histone and TAT, thus it can seize DNA and take it into cells. Now, it becomes our transfer device.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 260
  Illegal XhoI site found at 268
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 177
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 241