Plasmid

Part:BBa_K1437003

Designed by: Yifan Wu   Group: iGEM14_HIT-Harbin   (2014-09-26)


PRS304

This is a shuttle plasmid can not only growth in yeast can also grow in Escherichia coli,it has a Amp resistance and HIS auxotrophic.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1941
    Illegal XbaI site found at 549
    Illegal XbaI site found at 1911
    Illegal SpeI site found at 1917
    Illegal PstI site found at 1935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1941
    Illegal SpeI site found at 1917
    Illegal PstI site found at 1935
    Illegal NotI site found at 1903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1941
    Illegal BamHI site found at 1923
    Illegal XhoI site found at 1974
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1941
    Illegal XbaI site found at 549
    Illegal XbaI site found at 1911
    Illegal SpeI site found at 1917
    Illegal PstI site found at 1935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1941
    Illegal XbaI site found at 549
    Illegal XbaI site found at 1911
    Illegal SpeI site found at 1917
    Illegal PstI site found at 1935
    Illegal NgoMIV site found at 1562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3347
    Illegal SapI site found at 2264


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Categories
Parameters
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