Part:BBa_K1437001

PRS306 shuttle plasmid in yeast.

PRS306 is a shuttle plasmid which can express great both in E.coli and yeast.It has Amp resistance and it is URA auxotroph.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2047
  Illegal XbaI site found at 2017
  Illegal SpeI site found at 2023
  Illegal PstI site found at 389
  Illegal PstI site found at 2041
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2047
  Illegal SpeI site found at 2023
  Illegal PstI site found at 389
  Illegal PstI site found at 2041
  Illegal NotI site found at 2009
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2047
  Illegal BamHI site found at 2029
  Illegal XhoI site found at 2080
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2047
  Illegal XbaI site found at 2017
  Illegal SpeI site found at 2023
  Illegal PstI site found at 389
  Illegal PstI site found at 2041
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2047
  Illegal XbaI site found at 2017
  Illegal SpeI site found at 2023
  Illegal PstI site found at 389
  Illegal PstI site found at 2041
  Illegal NgoMIV site found at 1668
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1079
  Illegal BsaI.rc site found at 3453
  Illegal SapI site found at 2370
  Illegal SapI.rc site found at 926