Composite

Part:BBa_K1435002

Designed by: Matthew Chan   Group: iGEM14_Queens_Canada   (2014-10-17)


N-terminal GFP w/ an N-terminal Intein

This part is a N-terminal GFP with an intein fused to it. In the presence of the C-terminal intein conjugated to the C-terminal GFP, the intein will auto catalytically splice itself out and ligate its exteins together, forming the functional GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None