Composite

Part:BBa_K1433007

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)

B0034-gp35-Terminator

Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination between attB and attP, the attachment sites on phage chromosome and host chromosome. This recombination results in the reverse of the sequence between attB and attP, changing the two sites to attL and attR at the meantime. This inversion can be reversible by appropriately controlling the conditional expression of integrase and an excisionase in Bxb1 named gp47 at certain ratio. We change start codon ATG to GTG for a appropriate expression quantity.

ZJU_int-xis-bplr.gif

Composition

  1. RBS: BBa_B0034, a strong RBS.
  2. gp35: a serine integrase in Mycobacterium phage Bxb1.
  3. terminator: BBa_B0015, a strong double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
    Illegal XhoI site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
    Illegal NgoMIV site found at 1204
    Illegal AgeI site found at 254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1312


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Categories
Parameters
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