Generator

Part:BBa_K143081

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)

P43-spoVG RFP expression construct


RFP gene under expresson of the P43 promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.


Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.


This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part BBa_K143051) as part of the Biofabricator subtilis project.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2054
    Illegal AgeI site found at 2166
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None