Generator

Part:BBa_K143080

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)

P43-gsiB RFP expression construct


RFP gene under expresson of the P43 promoter and gsiB RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.


Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.


This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and gsiB RBS (Combined part BBa_K143050) as part of the Biofabricator subtilis project.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2052
    Illegal AgeI site found at 2164
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None