Composite

Part:BBa_K143076

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)

B. subtilis AmyE locus RFP output promoter and RBS tester


Long: AmyE 3' Integration sequence(BBa_K143002) coupled to RFP protein coding region and the registry double terminator (part BBa_J04650).


The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.


The RFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None