Composite
YtvA-T

Part:BBa_K143066

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-26)

YtvA light receptor Protein - Terminator


YtvA light receptor protein (YtvA)(BBa_K143037) coupled to the double terminator (BBa_B0015).


YtvA can be used in conjunction with a sigma factor B specific promoter (BBa_K143010 or BBa_K143011), where the YtvA will act as a receiver for a blue light input to result in a Polymerases per second (PoPS) output.


The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.


The double terminator allows the YtvA to be easily incorporated into a closed transcriptional unit.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 406
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157
    Illegal SapI site found at 475


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None