Part:BBa_K143055
Promoter hyper-spank and RBS spoVG for B. subtilis
Inducible promoter hyper-spank(BBa_K143015) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.
Phyperspank-spoVG can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.
IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143033). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
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