Coding
Aad9

Part:BBa_K143031

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-16)

Aad9 Spectinomycin Resistance Gene


Aad9 is the spectinomycin resistance gene from Enterococcus faecalis#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both B. subtilis and E. coli including pDP870#2, pCOMT-Kan#3 and pIEF16s#4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488


References

<biblio>

  1. 1 pmid=1659306
  2. 2 pmid=16997985
  3. 3 pmid=15060042
  4. 4 pmid=16714443

</biblio>

[edit]
Categories
//cds/selectionmarker/antibioticresistance
//cds/enzyme
Parameters
None