Device

Part:BBa_K1430008

Designed by: Cedric Blötz   Group: iGEM14_Goettingen   (2014-10-01)

Tool to tag yeast proteins with mRFP


Tool to tag yeast proteins with GFP

Use this plasmid to insert your protein of interest in the BamH1 or BgIII restriction site. It will be tagged to mRFP. The plasmid selection is done by leucine auxotrophy thanks to the LEU2 gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2313
    Illegal BamHI site found at 2322
    Illegal BamHI site found at 3342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 750
    Illegal AgeI site found at 2884
    Illegal AgeI site found at 2996
    Illegal AgeI site found at 3330
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aTool to tag yeast proteins with mRFP