Device

Part:BBa_K1430007

Designed by: Cedric Blötz   Group: iGEM14_Goettingen   (2014-10-01)

Tool to tag yeast proteins with GFP

Use this plasmid to insert your protein of interest in the BamH1 or BgIII restriction site. It will be tagged to GFP. The plasmid selection is done by tryptophan auxotrophy thanks to the TRP1 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1893
    Illegal BamHI site found at 1902
    Illegal BamHI site found at 2961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2553


[edit]
Categories
Parameters
n/aTool to tag yeast proteins with GFP