Device
Part:BBa_K1430007
Designed by: Cedric Blötz Group: iGEM14_Goettingen (2014-10-01)
Tool to tag yeast proteins with GFP
Use this plasmid to insert your protein of interest in the BamH1 or BgIII restriction site. It will be tagged to GFP. The plasmid selection is done by tryptophan auxotrophy thanks to the TRP1 gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1893
Illegal BamHI site found at 1902
Illegal BamHI site found at 2961 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2949
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2553
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Categories
Parameters
n/a | Tool to tag yeast proteins with GFP |