Coding

Part:BBa_K1430001

Designed by: Cedric Blötz   Group: iGEM14_Goettingen   (2014-09-30)

yeast LEU2 gene

Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene.

What does the marker help synthesize? L-leucine

Where it can be used? In LEU2 deficient strains of S. cerevisiae

Can it be used in different yeasts? yes - This can complement leu1- S. pombe, but mulitple copies are required.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None