Translational_Unit

Part:BBa_K1429003

Designed by: Eric Fernandez   Group: iGEM14_Genspace   (2014-10-02)

Orange Fluorescent Protein (OFP) "Yukon" coding region with RBS, intellectual property-free

IP free synthetic non-Aequorea orange fluorescent protein with ribosome binding site.


Usage and Biology

Can be used as a reporter. Production of OFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "OFP2":

UV_light.jpg

20141015_174513.jpg

The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


[edit]
Categories
Parameters
None