Part:BBa_K1429002

Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free

IP free synthetic non-Aequorea cyan fluorescent protein with ribosome binding site.

Usage and Biology

Can be used as a reporter. Production of CFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "CFP1":

The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 74
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2