RNA

Part:BBa_K1427001

Designed by: Connor McBrine   Group: iGEM14_Tufts_   (2014-10-17)


merRNA, c-di-GMP aptamer with promoter and terminators

This part contains BBa_K1427001 under the transcriptional control of a constitutive T7 promoter. Additionally, terminators developed by the Voigt Lab flank the merRNA sequence. There is an upstream ECK120033737 terminator which stops read-through by other genes. Downstream of the merRNA sequence, bidirectional termination is ensured with ECK120029600 and ECK120033736. It is likely these are redundant, as the poly-U tail of the merRNA suggests that is contains an intrinsic terminator.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 474
    Illegal NheI site found at 502
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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