RNA

Part:BBa_K1427000

Designed by: Connor McBrine   Group: iGEM14_Tufts_   (2014-10-09)

merRNA, c-di-GMP aptamer

The merRNA (massively expressed regulatory RNA) is a long, non-coding RNA transcript found in Bdellovibrio bacteriovorus which was described by Karnuker et al 2013. The 445 nt transcript contains a cyclic-di-GMP riboswitch sequence and is believed to mediate a change from growth phase to attack phase in Bdellovibrio by sequestering the second messenger c-di-GMP. When expressed constitutively under a T7 promoter in K12 E. coli, the merRNA increased biofilm formation. This is thought to be the result of the merRNA binding to and sequestering c-di-GMP molecules. This causes an increase in motility and flagella expression. The additional flagella adhere to surfaces and other cells, thereby forming a robust biofilm. Teams can insert this part directly downstream of a promoter sequence to cause stronger biofilm formation.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
    Illegal NheI site found at 426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None