Part:BBa_K1423004

CAEV capsid protein anchored in cell membrane

This part is a constitutive promoter, a ribosome binding site, a cell surface anchoring motif, a caprine arthritis encephalitis virus capsid protein domain, and a double terminator. We designed this part to use in our direct agglutination assay. The BCLA NTD will allow the capsid protein domain to be expressed on the outer surface of the cell. The capsid protein domain is an antigen which we then used with antibodies for agglutination.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

This construct has not been successful for agglutination at this time. This is a schematic of the construct that we made. It shows the origin of the N-terminal domain of BclA being combined with the CAEV capsid protein to form a plasmid then this plasmid being taken up by E. coli resulting in cell surface expression.