Composite

Part:BBa_K1422504

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-17)


Lactose-induced Killswitch using antiholin Hyb5

This is a killswitch designed for Bacillus subtilis. It is lactose and IPTG inducible, has a RBS that has high efficiency with Bacillus subtilis, the antiholin Hyb5 as a lysing agent, and a forward terminator. Hyb5 was chosen because it is effective on Bacillus subtilis at neutral pH and 37 Celsius. It is also effective on certain strains of E. coli and other bacteria. Source: Wang, S.; Kong, J.; & Zhang, X. (19 November, 2008). Identification and characterization of the two-component cell lysis cassette encoded by temperate bacteriophage ϕPYB5 of Lactobacillus fermentum. Journal of Applied Microbiology, Volume 105, Issue 6, pages 1939-1944. DOI: 10.1111/j.1365-2672.2008.03953.x http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03953.x/full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 457
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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