Protein_Domain

Part:BBa_K1422500

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-13)

Zea mays S-adenosylmethione synthase (corn SAM synthase)

S-adenosylmethione synthase (SAM synthase) alters L-methionine to have an adenosyl bonded to a sulfer on the L-methionine, producing S-adenosylmethione (SAM). This is the first enzyme in the sequence to produce corn phytosiderophores. BBa_K1422501, BBa_K1422502, and BBa_K1422503 are also required to produce a corn phytosiderophore. Note: This sequence has been codon optimized for Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 374
    Illegal PstI site found at 860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 820
    Illegal PstI site found at 374
    Illegal PstI site found at 860
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 310
    Illegal XhoI site found at 1194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 374
    Illegal PstI site found at 860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 374
    Illegal PstI site found at 860
    Illegal NgoMIV site found at 198
    Illegal NgoMIV site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1197


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Categories
Parameters
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