Device

Part:BBa_K1421002

Designed by: Mengni Wang   Group: iGEM14_CAU_China   (2014-10-04)


plux R+RBS+cI+2TE

This device is comprised of plux R (BBa_R0062), RBS (BBa_B0034), cI (Part:BBa_C0051) and 2 TE (BBa_B0010 and BBa_B0012). The lux pR is a right promoter in the lux cassette of V. Fischeri, which gives weak constitutive expression while enhanced when binding to the complex of LuxR and AHL (acyl-homoserine lactone ). CI is from BBa_C0051 which is designed by Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross. The cI repressor from E. coli phage lambda is modified with an LVA tail for rapid degradation of the protein. It binds to the cI regulator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None