Device

Part:BBa_K142047

Designed by: Julius Rabl   Group: iGEM08_ETH_Zurich   (2008-10-28)


tet-controlled LacI generator with constitutive TetR expression cassette

This BioBrick contains a tetracyclin-inducable LacI generator, which is followed by a constitutive TetR expression cassette. Since E. coli does not produce TetR, the expression cassette has to be present on the plasmid; otherwise the tet-controlled promotor will be constitutively active.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1385
    Illegal NheI site found at 1408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/regulation/transcriptional
Parameters
None