Device

Part:BBa_K1419001

Designed by: Zak Stinson   Group: iGEM14_Lethbridge   (2014-10-09)


Arabinose inducible lysis casette with RNA-IN

Identical to K1419000 but with the addition of an S32 RNA-IN sequence near the RBS for T4 Holin

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2572
    Illegal NheI site found at 2595
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2177
    Illegal AgeI site found at 2247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 2828


[edit]
Categories
Parameters
None