Generator

Part:BBa_K1418050

Designed by: Ryan Putman   Group: iGEM14_Utah_State   (2014-10-08)

Inducible PHB generator

An inducible promoter (BBa_K208010) was added in front of the phaCAB operon to allow for inducible bioplastic production.

Nmr.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1144
    Illegal BglII site found at 1969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 450
    Illegal NgoMIV site found at 521
    Illegal NgoMIV site found at 1121
    Illegal NgoMIV site found at 1433
    Illegal NgoMIV site found at 1712
    Illegal NgoMIV site found at 2364
    Illegal NgoMIV site found at 2386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4230


[edit]
Categories
Parameters
None