Plasmid

Part:BBa_K1416002

Designed by: Michael Hammerling, Razan Alnahhas, Jordan Monk   Group: iGEM14_Austin_Texas   (2014-10-17)

pStG: a ncAA Synthetase/tRNA Pair Plasmid Standard

A plasmid standard used in the 2014 UT Austin iGEM team's Expanded Genetic Code Measurement Kit. The plasmid contains a non-canonical amino acid synthetase and tRNA pair, in this case incorporating the canonical amino acid o-(2-nitrobenzyl)-L-tyrosine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1784
    Illegal XbaI site found at 3208
    Illegal PstI site found at 3613
    Illegal PstI site found at 3637
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1784
    Illegal NheI site found at 2366
    Illegal PstI site found at 3613
    Illegal PstI site found at 3637
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1784
    Illegal BglII site found at 1353
    Illegal BglII site found at 4395
    Illegal BamHI site found at 4401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1784
    Illegal XbaI site found at 3208
    Illegal PstI site found at 3613
    Illegal PstI site found at 3637
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1784
    Illegal XbaI site found at 3208
    Illegal PstI site found at 3613
    Illegal PstI site found at 3637
    Illegal NgoMIV site found at 85
    Illegal NgoMIV site found at 664
    Illegal NgoMIV site found at 3565
    Illegal NgoMIV site found at 4427
    Illegal NgoMIV site found at 4887
    Illegal NgoMIV site found at 5047
    Illegal AgeI site found at 489
    Illegal AgeI site found at 944
    Illegal AgeI site found at 2452
    Illegal AgeI site found at 2776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3558
    Illegal SapI.rc site found at 4351


[edit]
Categories
Parameters
None