![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K1416002
Designed by: Michael Hammerling, Razan Alnahhas, Jordan Monk Group: iGEM14_Austin_Texas (2014-10-17)
pStG: a ncAA Synthetase/tRNA Pair Plasmid Standard
A plasmid standard used in the 2014 UT Austin iGEM team's Expanded Genetic Code Measurement Kit. The plasmid contains a non-canonical amino acid synthetase and tRNA pair, in this case incorporating the canonical amino acid o-(2-nitrobenzyl)-L-tyrosine.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1784
Illegal XbaI site found at 3208
Illegal PstI site found at 3613
Illegal PstI site found at 3637 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1784
Illegal NheI site found at 2366
Illegal PstI site found at 3613
Illegal PstI site found at 3637 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1784
Illegal BglII site found at 1353
Illegal BglII site found at 4395
Illegal BamHI site found at 4401 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1784
Illegal XbaI site found at 3208
Illegal PstI site found at 3613
Illegal PstI site found at 3637 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1784
Illegal XbaI site found at 3208
Illegal PstI site found at 3613
Illegal PstI site found at 3637
Illegal NgoMIV site found at 85
Illegal NgoMIV site found at 664
Illegal NgoMIV site found at 3565
Illegal NgoMIV site found at 4427
Illegal NgoMIV site found at 4887
Illegal NgoMIV site found at 5047
Illegal AgeI site found at 489
Illegal AgeI site found at 944
Illegal AgeI site found at 2452
Illegal AgeI site found at 2776 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3558
Illegal SapI.rc site found at 4351
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Categories
Parameters
None |