Part:BBa_K1415205

Pcons+B0034+PBAN(Spodoptera litura)+B0034+BFP+J61048

Fig.1-1 Biobrick of P_cons + RBS + PBAN(Spodoptera litura ) + RBS + BFP + Ter.

To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of P_cons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli. Below is the blue fluorescence expression curve and bacterial growth curve (OD 600) in a long period of time. We used these data to predict the PBAN expression in E.coli.

Fig 1-2 Blue Fluorescence of P_cons + RBS + PBAN(Spodoptera litura) +RBS + BFP + Ter.

Fig.1-3-1 The blue light fluorescence expression curve of E.coli containing Pcons + RBS + PBAN(HAH) + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light).

Fig.1-3-2 The growth curves of PBAN(SL)

Modeling

Fig.1-5 Modeling result of P_cons + RBS + PBAN(Spodoptera litura) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of P_cons + RBS + PBAN(Spodoptera litura) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.

The device we design and working mechanism

Fig.1-6 Our Project Overview.

In our project, we will biologically synthesize PBAN with our E.coli. We store the PBAN inside a trapping device. In the device, there will be appropriate lighting and nutrient sources that will attract insects.

Once an insect is attracted into our device and ingests the nutrient sources we provide, it will also inevitably come in contact with our PBAN. As the PBAN works and activates the pheromone synthesis of the attracted insect, more of this species of insect’s counterparts will be attracted and later captured.

Owing to the first feature mentioned above, PBAN are species-specific, which means that it doesn't matter if other kind of insect fly into our device and eat PBAN, because the insects we don't want to catch will not be stimulated by PBANs to produce pheromone; our PBAN are only for what we want to catch, and we are sure that our method won't affect other kinds of insects.