Composite

Part:BBa_K1415203

Designed by: HO, TSUNG YU   Group: iGEM14_NCTU_Formosa   (2014-10-05)

Pcons+B0034+PBAN(Agrotis ipsilon)+B0034+BFP+J61048

We use this part as reporter gene, it can check whether our PBAN works and quantify its expression.

[[File:Pcons(J23101) + RBS(B0034) + PBAN(AI) + RBS(B0034) + BFP(K592100) + Ter(J61048) .png|thumb|center|600px|Fig.1-1 Biobrick of Pcons + RBS + PBAN(AI) + BFP + Term.]

Fig.1-2 Fig.1-2 The bluelight test of our E.coli


Fig.1-3 The blue light fluorescence expression curve of E.coli containing Pcons + RBS + 9 different kinds of PBAN + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light).　Below are biobrick serial numbers of the PBAN abbreviations: SL: BBa_K1415205　　　　BM: BBa_K1415201　　　　MB: BBa_K1415202 AI: BBa_K1415203　　　　LD: BBa_K1415204　　　　HAH:BBa_K1415206 AS: BBa_K1415207　　　　SI: BBa_K1415208　　　　AA: BBa_K1415209


Modeling

Fig.1-4 Modeling result of Pcons + RBS + PBAN(AI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 909
  • 1000
    COMPATIBLE WITH RFC[1000]


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