Part:BBa_K1415201

Pcons+B0034+PBAN(Bombyx mori)+B0034+BFP+J61048

Fig.1-1 Biobrick of P_cons + RBS + PBAN(Bombyx mori ) + RBS + BFP + Ter.

To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of P_cons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli. Below is the blue fluorescence expression curve and bacterial growth curve (OD 600) in a long period of time. We used these data to predict the PBAN expression in E.coli.

Fig.1-2 Blue Fluorescence of P_cons + RBS + PBAN(Bombyx mori) +RBS + BFP + Ter.

Fig.1-3 The blue light fluorescence expression curve of E.coli containing Pcons + RBS + 9 different kinds of PBAN + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light).　Below are biobrick serial numbers of the PBAN abbreviations: SL: BBa_K1415205　　　　BM: BBa_K1415201　　　　MB: BBa_K1415202 AI: BBa_K1415203　　　　LD: BBa_K1415204　　　　HAH:BBa_K1415206 AS: BBa_K1415207　　　　SI: BBa_K1415208　　　　AA: BBa_K1415209

Modeling

Fig.1-4 Modeling result of P_cons + RBS + PBAN(Bombyx mori) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of P_cons + RBS + PBAN(Bombyx mori) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 909
1000
COMPATIBLE WITH RFC[1000]