Not Released
Experience: Works
Not Used
Get This Part
Composite

Part:BBa_K1415103

Designed by: HO, TSUNG YU   Group: iGEM14_NCTU_Formosa   (2014-10-05)

Pcons+B0034+PBAN(Agrotis ipsilon)

This part has a constitutive promoter, so our PBAN(Agrotis ipsilon) can constitutive express.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Fig.1-1 Biobrick of Pcons + RBS + PBAN(AI)
Fig.1-2 Protein Electrophoresis of Pcons + RBS + PBAN(AI) (control: plasmid of Pcons+RBS) Each peptide of PBAN is an around 30 amino acids, so we can see the band of PBANs at 2~4 kDa.
[edit]
Categories
Parameters
None