Not Released
Sample In stock
Experience: Works
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Composite

Part:BBa_K1415101

Designed by: HO, TSUNG YU   Group: iGEM14_NCTU_Formosa_   (2014-10-03)

Pcons+B0034+PBAN(Bombyx mori)

This part has a constitutive promoter, so our PBAN(Bombyx) can constitutive express.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


Fig.1-1 Biobrick of Pcons + RBS + PBAN(BM)
Fig.1-2 Protein Electrophoresis of Pcons + RBS + PBAN(BM) (control: plasmid of Pcons+RBS) Each peptide of PBAN is an around 30 amino acids, so we can see the band of PBANs at 2~4 kDa.
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Categories
Parameters
n/aPcons+B0034+PBAN(Bombyx mori)