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Part:BBa_K1413022

Designed by: Laura MATABISHI   Group: iGEM14_Evry   (2014-10-17)


J23114-B0034-bphR2 mutated-B0015

bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, is involved in the transcription of bphR1 gene which activates the genes of degradation of biphenyls. bphR2 has been mutated to remove the pstI site in its sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 522
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 306


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